To determine diversity of endophytic fungi associated with the lianas Combretum roxburghii from different forests of West Medinipur district. Samples were collected randomly in various seasons. Fungi were isolated and identified based on mycelial shape and structure; sexual and asexual reproductive characters; attachment of spores and cultural conditions. A total of 603 endophytic fungi were isolated from 675 different sample segments. Colonization frequency is 74.66%. In Belpahari highest number of endophytes have been isolated and Chilkigarh was also nearer to Belpahari. In summer highest percentage (92.44%) were isolated. Dominant endophytic fungi were Verticillium, Pestalotiopsis, Beltrania, Chaetomium. Maximum endophytic isolates were obtained from stem segments followed by leaves and petioles. Among all endophytic fungi class Deuteromycetes were dominant over other fungi classes. Shannon-Weiner and Simpson's indices showed rich diversity of endophytic fungi. This indices suggest even and uniform occurrence of various species.