Fungal endophytes are microfungi that live within various tissues of plants without\r\ncausing apparent symptoms of infection. This study was conducted to identify the endophytic fungi isolated from leaf petiole and stem of Bauhinia vahlii from three different localities of West Medinipur district. A total 300 isolates were obtained from 375 sample segments. The isolated fungi belong to 38 genera with a few unknown genera and sterile mycelial fungi. Among all fungi Penicillium sp. (19.57%) Pestalotiopsis sp. (12.17%) and Aspergillus sp.(10.43%) were most commonly found. Phialophora sp. Nigrospora sp. Torula sp. Bispora sp. Curvularia sp. were also isolated. The colonization frequency (CF) is much higher in petiole (86.67%) in comparison to stem (77.33%) and leaf (70.67%). This study showed the tissue specificity of endophytic symbiosis. There was a variation of CF in respect of localities. Endophytic fungal diversity was higher in plants of Chilkigarh.\r\nKeywords: Bauhinia vahlii endophyte diversity forest lianas fungi.