The objective was to evaluate the seed bank of three different areas under restoration process after 12, 13 and 16 years of implantation, located in the state of Mato Grosso do Sul. Twenty samples (20cm x 20cm) were collected inside each forest restored in randomly distributed points, at a depth of 0 to 5cm, considering the litter. After being placed in plastic trays, the samples were irrigated and monitored daily for a period of approximately three months (90 days). The species were also evaluated according to their dispersal syndrome, adopting the morphological criteria of the fruits as anemocoric, zoochoric, and autocoric, besides being classified according to the way of life and origin. Seed bank diversity was estimated through Shannon diversity index (H ') and Pielou Equity (J'). These analyzes were performed using the Fitopac 2.0 program. It was found that the composition of the herbaceous community varied with the localities, being the highest viable seed density for this class observed in Ivinhema, where the seed bank was composed mainly of spontaneous herbs from anthropized areas around the area and high density of seeds. seedlings of the Poaceae family, with four distinct species. The Jateí area presented a high density of Cecropia pachystachyaTrécul. Seedlings, as well as four other distinct tree species. The restored area of Caarapó presented different vegetation classes and the presence of important succession components such as trees and vines. In the three seed banks evaluated, there was the presence of tree species representing an advance in the successional process of each area.